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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
06/11/2002 |
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Data da última atualização: |
06/11/2002 |
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Autoria: |
RUEDA, B. R.; HENDRY, I. R.; TILLY, J. L.; HAMERNIK, D. L. |
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Título: |
Accumulation of caspase-3 messenger ribocleic acid and induction of caspase activity in the ovine corpus luteum following prostaglandin Falpha treatment in vivo. |
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Ano de publicação: |
1999 |
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Fonte/Imprenta: |
Biology of Reproduction, v. 60, n. 5, p. 1087-1092, 1999. |
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Idioma: |
Inglês |
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Conteúdo: |
Caspase-3, a vertebrate homologue of the protein encoded by the Caenorhabditis elegans cell death gene, ced-3, induces apoptosis when overexpressed in eukaryotic cells. Since apoptosis occurs during corpus luteum (CL) regression in many species, including the ewe, these studies were conducted to 1) isolate a cDNA encoding ovine caspase-3, 2) measure steady state amounts of caspase-3 mRNA in the CL during luteolysis induced by prostaglandin F2alpha (PGF2alpha) and during the time of maternal recognition of pregnancy, and 3) measure changes in caspase activity during PGF2alpha-initiated luteal regression. Oligonucleotide primers corresponding to a human caspase-3 cDNA sequence were combined with total RNA from ovine CL in a reverse transcription-polymerase chain reaction-based procedure to amplify a 640-base pair partial cDNA with a nucleotide sequence 86% and 81 % identical to the human and rat caspase-3 cDNAs, respectively. CL were collected from ewes at 0, 12, or 24 h after treatment with PGF2alpha on Day 10 of the estrous cycle and from nonpregnant and pregnant ewes on Day 12 or Day 14 of the cycle. Northern blot analysis of total cellular RNA from ovine CL and a radiolabeled ovine caspase-3 cRNA probe indicated the presence of a single mRNA transcript of approximately 2.5 kilobases. Levels of caspase-3 mRNA were approximately 3-fold higher (p < 0.05) in CL at 12 h and 24 h after PGF2alpha in comparison to those levels measured in matched CL from untreated ewes. There were no differences (p > 0.05) in amounts of caspase-3 mRNA in CL on Day 12 or Day 14 of the estrous cycle compared to Day 12 or Day 14 of pregnancy, respectively. Caspase activity in CL (measured by the ability of CL lysates to cleave an artificial caspase substrate) was also significantly (p < 0.05) increased in CL collected after treatment with PGF2alpha compared 1 to CL collected from nontreated ewes. We conclude that physiological cell death during PGF2a-induced luteal regression in the ewe is mediated, at least in part, via increased expression and activity of the caspase family of pro-apoptotic proteases. MenosCaspase-3, a vertebrate homologue of the protein encoded by the Caenorhabditis elegans cell death gene, ced-3, induces apoptosis when overexpressed in eukaryotic cells. Since apoptosis occurs during corpus luteum (CL) regression in many species, including the ewe, these studies were conducted to 1) isolate a cDNA encoding ovine caspase-3, 2) measure steady state amounts of caspase-3 mRNA in the CL during luteolysis induced by prostaglandin F2alpha (PGF2alpha) and during the time of maternal recognition of pregnancy, and 3) measure changes in caspase activity during PGF2alpha-initiated luteal regression. Oligonucleotide primers corresponding to a human caspase-3 cDNA sequence were combined with total RNA from ovine CL in a reverse transcription-polymerase chain reaction-based procedure to amplify a 640-base pair partial cDNA with a nucleotide sequence 86% and 81 % identical to the human and rat caspase-3 cDNAs, respectively. CL were collected from ewes at 0, 12, or 24 h after treatment with PGF2alpha on Day 10 of the estrous cycle and from nonpregnant and pregnant ewes on Day 12 or Day 14 of the cycle. Northern blot analysis of total cellular RNA from ovine CL and a radiolabeled ovine caspase-3 cRNA probe indicated the presence of a single mRNA transcript of approximately 2.5 kilobases. Levels of caspase-3 mRNA were approximately 3-fold higher (p < 0.05) in CL at 12 h and 24 h after PGF2alpha in comparison to those levels measured in matched CL from untreated ewes. There were... Mostrar Tudo |
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Thesagro: |
Corpo Lúteo; Endocrinologia; Hormônio; Ovino; Prostaglandina; Reprodução Animal. |
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Categoria do assunto: |
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Marc: |
LEADER 02827naa a2200229 a 4500
001 1528830
005 2002-11-06
008 1999 bl uuuu u00u1 u #d
100 1 $aRUEDA, B. R.
245 $aAccumulation of caspase-3 messenger ribocleic acid and induction of caspase activity in the ovine corpus luteum following prostaglandin Falpha treatment in vivo.
260 $c1999
520 $aCaspase-3, a vertebrate homologue of the protein encoded by the Caenorhabditis elegans cell death gene, ced-3, induces apoptosis when overexpressed in eukaryotic cells. Since apoptosis occurs during corpus luteum (CL) regression in many species, including the ewe, these studies were conducted to 1) isolate a cDNA encoding ovine caspase-3, 2) measure steady state amounts of caspase-3 mRNA in the CL during luteolysis induced by prostaglandin F2alpha (PGF2alpha) and during the time of maternal recognition of pregnancy, and 3) measure changes in caspase activity during PGF2alpha-initiated luteal regression. Oligonucleotide primers corresponding to a human caspase-3 cDNA sequence were combined with total RNA from ovine CL in a reverse transcription-polymerase chain reaction-based procedure to amplify a 640-base pair partial cDNA with a nucleotide sequence 86% and 81 % identical to the human and rat caspase-3 cDNAs, respectively. CL were collected from ewes at 0, 12, or 24 h after treatment with PGF2alpha on Day 10 of the estrous cycle and from nonpregnant and pregnant ewes on Day 12 or Day 14 of the cycle. Northern blot analysis of total cellular RNA from ovine CL and a radiolabeled ovine caspase-3 cRNA probe indicated the presence of a single mRNA transcript of approximately 2.5 kilobases. Levels of caspase-3 mRNA were approximately 3-fold higher (p < 0.05) in CL at 12 h and 24 h after PGF2alpha in comparison to those levels measured in matched CL from untreated ewes. There were no differences (p > 0.05) in amounts of caspase-3 mRNA in CL on Day 12 or Day 14 of the estrous cycle compared to Day 12 or Day 14 of pregnancy, respectively. Caspase activity in CL (measured by the ability of CL lysates to cleave an artificial caspase substrate) was also significantly (p < 0.05) increased in CL collected after treatment with PGF2alpha compared 1 to CL collected from nontreated ewes. We conclude that physiological cell death during PGF2a-induced luteal regression in the ewe is mediated, at least in part, via increased expression and activity of the caspase family of pro-apoptotic proteases.
650 $aCorpo Lúteo
650 $aEndocrinologia
650 $aHormônio
650 $aOvino
650 $aProstaglandina
650 $aReprodução Animal
700 1 $aHENDRY, I. R.
700 1 $aTILLY, J. L.
700 1 $aHAMERNIK, D. L.
773 $tBiology of Reproduction$gv. 60, n. 5, p. 1087-1092, 1999.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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| Registros recuperados : 26 | |
| 3. |  | MOURA, S. C. S. R. de; GERMER, S. P. M.; ANJOS, V. D. de A.; MORI, E. E. M.; MATTOSO, L. H. C.; FIRMINO, A.; NASCIMENTO, C. J. F. Avaliações físicas, químicas e sensoriais de blends de café arábica com café canephora (robusta) = Evaluation of the physical, chemical and sensory characteristics of arabica and canephora (robusta) coffee blends. Brazilian journal of food technology, Campinas, v. 10, n. 4 p. 271-277, 2007.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Nacional - A |
| Biblioteca(s): Embrapa Instrumentação. |
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| 6. | | LEITE, A. I. N.; VICENTINI, N. M.; ANJOS, V. dos; MEDEIROS, I. de F. Caracterização preliminar das principais regiões do espectro infravermelho do queijo de manteiga. In: CONGRESSO BRASILEIRO DE CIÊNCIA E TECNOLOGIA DE ALIMENTOS, 26., 2018, Belém, PA. O uso consciente da biodiversidade: perspectivas para o avanço da ciência e tecnologia de alimentos. Belém, PA: SBCTA:UFPA, 2018.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 7. | | TERAO, D.; NECHET, K. de L.; FRIGHETTO, R. T. S.; ANJOS, V. D. A.; MAIA, A. de H. N.; HALFELD-VIEIRA, B. de A. Control of Fusarium rot in Galia melon and preservation of fruit quality with UV-C radiation and hot water treatments. Tropical Plant Pathology, v. 46, n. 3, p. 350-359, 2021.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Meio Ambiente. |
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| 8. | | TOKIMATU, C. C.; CASTRO, M. F. P. P. M. de; SIGRIST, J. M. M.; ANJOS, V. D. A.; BENATO, E. A.; TERAO, D.; OLIVEIRA, S. B. de; VITALI, A. A.; VALENTINI, S. R. de T.; REZENDE, A. C. B.; PENTEADO, A. L.; SCHMIDT, F. L. Controle alternativo de Salmonella Enterica Serotype Enteritidis em manga (Mangifera indica L.) pós-colheita. In: CONGRESSO INTERINSTITUCIONAL DE INICIAÇÃO CIENTÍFICA, 5., 2011, Campinas. Anais... Campinas: Embrapa Monitoramento por Satélite, 2011. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroindústria de Alimentos. |
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| 9. | | SANTANA, W. M.; SOUSA, F. J. A.; ANJOS, V. M.; SOUSA, J. M. S.; CASTRO, P. F.; PEREIRA, A. M. L. Desempenho produtivo do camarão Litopenaeus vannamei cultivado em águas interiores sob diferentes estratégias alimentares. In: CONGRESSO BRASILEIRO DE OCEANOGRAFIA, 3.; CONGRESSO IBERO-AMERICANO DE OCEANOGRAFIA, 1., 2008, Fortaleza. Anais... Fortaleza: Associação Brasileira de Oceanografia, 2008. 3 p.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Meio-Norte. |
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| 10. | | POLICARPO, V. M.; ENDO, E.; FARIA, R. Q.; ANJOS, V.; BORGES, S. V.; GREGÓRIO, S. R.; CAVALCANTI, N. de B. Efecto de aditivos sobre el calor, textura y aceptación del dulce de umbu (Spondias tuberosa, Arr.Cam.) verde. Alimentaria, Madrid, n. 346, p. 111-116, 2003.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Internacional - B |
| Biblioteca(s): Embrapa Semiárido. |
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| 11. | | SILVA, D. J.; PETRERE, V. G.; MOUCO, M. A. do C.; GAVA, C. A. T.; SILVA, D. O. M. da; ANJOS, V. H. A. dos. Efeito de compostos orgânicos nas características de crescimento de mangueiras cultivadas em sistema orgânico de produção. In: SIMPÓSIO BRASILEIRO DE AGROPECUÁRIA SUSTENTÁVEL, 1., 2009, Viçosa, MG. Agricultura, pecuária e cooperativismo: anais de resumos expandidos. Viçosa, MG: Universidade Federal de Viçosa, 2009. p. 464-468. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Semiárido. |
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| 12. | | PEROBELLI, R.; SILVA, C. A. da; DEMARQUE, D.; MASELLI, M.; NOGUEIRA, J. S.; BELL, M. J. V.; SILVA, P. H. F. da; LANGE, C. C.; VICENTINI, N. M.; ANJOS, V. Espectroscopia ótica e de massa na caracterização de leite e derivados. In: COSTA, F. F.; HÚNGARO, H. M.; RODARTE, M. P. (org.). Gestão, qualidade e inovações tecnológicas: produções acadêmicas em ciência e tecnologia do leite e derivados. Juiz de Fora: Editar, 2023. v. 1. p. 191-217.| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 14. | | LANGE, C. C.; OLIVEIRA, R. P. de; SILVA, M. A. S.; PINTO, C. O.; VICENTINI, N. M.; BELL, M. J. V.; ANJOS, V. de C. dos. Identificação de leveduras isoladas de casos de mastite bovina por MALDI-TOF MS. In: CONGRESSO BRASILEIRO DE RECURSOS GENÉTICOS, 7., 2022. Multifuncionalidade e qualidade de vida: resumos. Brasília, DF: Sociedade Brasileira de Recursos Genéticos, 2022.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 16. | | LEITE, A. I. N.; VICENTINI, N. M.; COSTA, R. G. B.; ANJOS, V. C. dos; PEREIRA, C. G.; MEDEIROS, I. de F. Influência do teor de gordura na cor e perfil de textura do queijo de manteiga. In: CONGRESSO NACIONAL DE LATICÍNIOS, 32., 2019, Juiz de Fora. Resumos... Belo Horizonte: EPAMIG, 2019. p. 38.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 17. | | MOURA, S. C. S. R. de; GERMER, S. P. M.; ANJOS, V. D. de A.; MORI, E. E. M.; MATTOSO, L. H. C; FIRMINO, A.; NASCIMENTO, C. J. F. Influência dos parâmetros de torração nas características físicas, químicas e sensoriais do Café Arábia puro. Brazilian Journal of food technology, Campinas, v. 10, n. 1, p. 17-25, 2007.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Nacional - A |
| Biblioteca(s): Embrapa Instrumentação. |
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| 20. | | TERAO, D.; NECHET, K. de L.; FRIGHETTO, R. T. S.; ANJOS, V. D. A.; BENATO, E. A.; HALFELD-VIEIRA, B. de A. Physical postharvest treatments in the control of stem-end rot of mango. Journal of Phytopathology, v. 166, n. 7-8, p. 581-589, 2018.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Meio Ambiente. |
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| Registros recuperados : 26 | |
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